ANTAGONISTIC TEST OF RUBBER PLANT RHIZOSPHERE FUNGAL ISOLATES AT PT. BRIDGESTONE KALIMANTAN PLANTATION (BSKP) AGAINST Pestalotiopsis sp. IN VITRO

Yenny Nur Syahda Oktaviani; Mariana Mariana; Salamiah Salamiah; Yusriadi Marsuni; Helda Orbani Rosa; Muhammad Indar Pramudi

Yenny Nur Syahda Oktaviani Student of Plant Protection, Agriculture Faculty, Lambung Mangkurat University
Mariana Mariana Plant Protection, Agriculture Faculty, Lambung Mangkurat University
Salamiah Salamiah Plant Protection, Agriculture Faculty, Lambung Mangkurat University
Yusriadi Marsuni Plant Protection, Agriculture Faculty, Lambung Mangkurat University
Helda Orbani Rosa Plant Protection, Agriculture Faculty, Lambung Mangkurat University
Muhammad Indar Pramudi Plant Protection, Agriculture Faculty, Lambung Mangkurat University

Keywords: Antagonistic Fungi, Hevea brasiliensis, Pestalotiopsis sp, PT BSKP

Abstract

Rubber plants (Hevea brasiliensis) are plantation commodities that play an important role in the Indonesian economy and are the main foreign exchange earner in the plantation sector. In the rubber company Bridgestone Kalimantan Plantation (BSKP), leaf fall disease is caused by the pathogen Pestalotiopsis sp. Control techniques using excessive chemical pesticides can damage the balance of the ecosystem and pollute the environment. One environmentally friendly alternative control is antagonistic fungi that can suppress the growth of pathogens that cause disease. The purpose of this study was to determine the ability of antagonistic fungi from the rhizosphere of rubber plants at PT BSKP to inhibit Pestalotiopsis sp. This study was conducted at the Phytopathology Laboratory of Lambung Mangkurat University. Sampling was carried out at the rubber plantation of PT BSKP, Tanah Laut Regency, South Kalimantan. Samples were taken from 4 plots where and each plot consisted of 5 subplots (5000 m2). From each subplot, 1 point/tree was taken. The method was a Completely Randomized Design (CRD) with 8 treatments (antagonistic isolates) and 3 replications. The results of the isolation of rubber plant rhizosphere fungi obtained 15 pure isolates which were then selected by pathogenicity test and received 7 pathogenic isolates and 8 non-pathogenic isolates. Furthermore, an inhibition test was carried out to determine the ability of antagonistic fungi, the results of this test contained 2 isolates, namely B3T7 and B2T1 which had an inhibition percentage of >50%, respectively, 66.01% and 51.78%. In addition, isolate B1T2 with an inhibition percentage of 29.39% has an antibiosis mechanism that can also be categorized as an antagonist. Further identification by morphological observation showed that isolate B3T7 was Trichoderma sp., isolate B2T1 was Purpureocillium sp. and isolate B1T2 was Penicillium sp.

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